Application: This product is for research use only and is not intended for diagnostic use.

Features: Calculation
If samples generate values ​​higher than the highest standard, dilute the samples and repeat the assay.
Calculate the mean absorbance for each standard, control and sample and subtract average zero standard optical density (O.D.).
Construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph. Most graphing software can help make the curve and a four parameter logistic (4-PL) usually provide the best fit, though other equations (eg linear, log / log) can also be tried to see which provides the most accurate. Extrapolate the target protein concentrations for unknown samples from the standard curve plotted.

Sensitivity & Specificity
The sensitivity of this kit is 6.25 ng / mL.
This kit have been tested for SARS-CoV-2 Nucleoprotein, and has no react with recombinant MERS-CoV Nucleoprotein.

Reproducibility
The assay was repeated 10 times with a CV less than 15%.
Three lots were tested with the same samples 10 times with a CV less than 15%.

Limitations
1. This product is only used for testing of serum or plasma samples.
2. The test results of this product are for clinical reference only, and they should not be used as the sole basis for the diagnosis of new coronavirus.
3. Due to the window effect of the virus infection and the sensitivity of the kit detection, a negative test result cannot exclude the possibility of virus infection.

The new Coronavirus (SARS-COV-2) antigen detection kit is based on the principle of a double antibody sandwich enzyme- linked immunoassay. The microplate is pre-coated with an anti-new coronavirus N protein antibody. When the serum and plasma to be detected contain the new coronavirus N protein antigen, it will bind to the specific antibody coated on the microplate. After the secondary antibody against the new coronavirus N protein was added, an "antibody-antigen-enzyme- labeled antibody" complex is formed. After adding the chromogenic substrate TMB, HRP enzyme will catalyze the color development. After terminating the solution, the microplate reader will determine the Absorbance value to determine the presence or absence of new coronavirus antigen in each test sample.